SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
Solved The annealing temperature of a DNA duplex is given by | Chegg.com
How low must be the annealing temp of the first rounds for overhang pcr? | ResearchGate
Design and Applications in Molecular Biology Research: Primer Design - ppt video online download